![]() ![]() ![]() In conclusion, CAXPCi holds a strong potential for being adopted as a routine laboratory tool for non-destructive, high throughput assessment of 3D structural changes in murine articular cartilage, with a possible impact in the field similar to the revolution that conventional microCT brought into bone research. Histochemical visualization of the cartilage hyaladherins using a biotinylated hyaluronan oligosaccharide bioaffinity probe. From a technological standpoint, by showing the capability of the system to detect cartilage also in water, we demonstrate phase sensitivity comparable to other lab-based phase methods (e.g. Cartilage contact stress in retroverted hips for all subjects and loading scenarios. The fringe plots shown in the walking mid, descending stairsand chair rise rows were averaged to create Figure 5. Following these successful proof-of-concept results in rat cartilage, we expect that an upgrade of the system to higher resolutions (currently underway) will enable extending the method to the imaging of mouse cartilage as well. Cartilage contact patterns were subject-specific, but tended to be fairly distributed over the articular surface. Read more related scholarly scientific articles and abstracts. Moreover, we show that small, surgically induced lesions are also correctly detected by the CAXPCi system, and we support this finding with histopathology examination. The total knee joint cartilage volume ranged from 16.6 to 31.4 ml, the size of the articular surfaces from 102 to 163 cm(2), and the mean cartilage thickness from 1.57 to 2.43 mm. Histochemical visualization of the cartilage hyaladherins using a biotinylated hyaluronan oligosaccharide bioaffinity probe. We show that a simple laboratory system based on coded-aperture x-ray phase contrast imaging (CAXPCi) can correctly visualize the cartilage layer in slices of an excised rat tibia imaged both in air and in saline solution. ![]() Following these successful proof-of-concept results in rat cartilage, we expect that an upgrade of the system to higher resolutions. Marenzana M(1), Hagen CK, Das Neves Borges P, Endrizzi M, Szafraniec MB, Ignatyev K, Olivo A. However, due to limitations in imaging technology, high-throughput 3D structural imaging has not been achievable in small rodent models, thereby limiting their translational potential and their efficiency as research tools. Visualization of small lesions in rat cartilage by means of laboratory-based x-ray phase contrast imaging. These models are becoming essential tools for the development of new drugs for OA, a disease affecting up to 1/3 of the population older than 50 years for which there is no cure except prosthetic surgery. The right functioning of natural synovial joints ensures well lubricated contact surfaces with a very low friction coefficient and wear of cartilage tissue. Being able to quantitatively assess articular cartilage in three-dimensions (3D) in small rodent animal models, with a simple laboratory set-up, would prove extremely important for the development of pre-clinical research focusing on cartilage pathologies such as osteoarthritis (OA). ![]()
0 Comments
Leave a Reply. |